Bhandari Lab Faculty

Principal Investigator

Vineet Bhandari, MD, DM, FAAP

Vineet Bhandari, MD, DM, FAAP

Prinicipal Investigator

About Me

My research in the laboratory is on pulmonary developmental pathology, with a primary focus on hyperoxia-induced injury and resolution/repair in developing lungs. Using a variety of ante- and/or post-natal animal models of experimental bronchopulmonary dysplasia (BPD) and hyperoxia-induced acute lung injury (HALI), we study the mechanisms of molecular mediators and signaling pathways of inflammation, alveolarization and vascularization contribution to the disease phenotypes of HALI and BPD. The ultimate aim of the research laboratory is to be able to translate our work into a better understanding of the pathogenesis of HALI and BPD to allow improved targeting of therapeutic interventions. We have long-standing collaborations with physicians and scientists from various Universities and Pharmaceutical companies. Our long-term goal is to identify drug candidates for HALI and BPD. My other research interests include Neonatal Sepsis, BPD-associated Pulmonary Hypertension and use of “OMICS” (genomics, transcriptomics, proteomics, metabolomics and microbiomics) approaches relevant to premature neonates.

Publications

Das P, Curstedt T, Agarwal B, Prahaladan VM, Ramirez J, Bhandari S, Syed MA, Salomone F, Casiraghi C, Pelizzi N, Bhandari V. Small Molecule Inhibitor Adjuvant Surfactant Therapy Attenuates Ventilator- and Hyperoxia-Induced Lung Injury in Preterm Rabbits. Front Physiol. 2020 Apr 9;11:266. doi: 10.3389/fphys.2020.00266. eCollection 2020.

Gilfillan M, Das P, Shah D, Alam MA, Bhandari V. Inhibition of microRNA-451 is associated with increased expression of Macrophage Migration Inhibitory Factor and mitigation of the cardio-pulmonary phenotype in a murine model of Bronchopulmonary Dysplasia. Respir Res. 2020 Apr 22;21(1):92. doi: 10.1186/s12931-020-01353-9.

Alam MA, Betal SGN, Aghai ZH, Bhandari V. Hyperoxia causes miR199a-5p-mediated injury in the developing lung. Pediatr Res. 2019 Nov;86(5):579-588. doi: 10.1038/s41390-019-0524-3. Epub 2019 Aug 8.

For a complete list, please see:
https://www.ncbi.nlm.nih.gov/pubmed/?term=bhandari%2C+vineet

Scientists

Pragnya Das

Pragnya Das

About Me

Senior Scientist, Bhandari Lab
das-pragnya@cooperhealth.edu

My research interests include studying transcriptional regulation during fetal lung development, role of long and short noncoding RNAs, ion channels and post transcriptional regulation of genes during lung development. We have recently identified a novel small molecule immunomodulator that can be targeted towards preventing BPD in neonatal mouse models. We have also shown that small molecular inhibitors can be used as therapeutic targets in association with surfactant, in a lung injury model of preterm rabbits.

Publications

Das P, Curstedt T, Agarwal B, Prahaladan VM, Ramirez J, Bhandari S, Syed MA, Salomone F, Casiraghi C, Pelizzi N, Bhandari V. Small Molecule Inhibitor Adjuvant Surfactant Therapy Attenuates Ventilator- and Hyperoxia-Induced Lung Injury in Preterm Rabbits. Front Physiol. 2020 Apr 9;11:266. doi: 10.3389/fphys.2020.00266. eCollection 2020.
PMID: 32327998

Das P, Panda SK, Agarwal B, Behera S, Ali SM, Pulse ME, Solomkin JS, Opal SM, Bhandari V, Acharya S. Novel Chitohexaose Analog Protects Young and Aged mice from CLP Induced Polymicrobial Sepsis. Sci Rep. 2019 Feb 27;9(1):2904. doi: 10.1038/s41598-019-38731-3.
PMID: 30814582

Leary S*, Das P*, Ponnalagu D, Singh H, Bhandari V.Genetic Strain and Sex Differences in a Hyperoxia-Induced Mouse Model of Varying Severity of Bronchopulmonary Dysplasia. Am J Pathol. 2019 May;189(5):999-1014. doi: 10.1016/j.ajpath.2019.01.014. Epub 2019 Feb 19.
PMID: 30794808

Das P, Syed MA, Shah D, Bhandari V.miR34a: a master regulator in the pathogenesis of bronchopulmonary dysplasia. Cell Stress. 2018 Jan 9;2(2):34-36. doi: 10.15698/cst2018.02.1224.
PMID: 31225464

For a complete list, please see:
https://www.ncbi.nlm.nih.gov/pubmed/?term=Pragnya+Das

Dilip Shah

Dilip Shah

About Me

Senior Scientist, Bhandari Lab
shah-dilip@cooperhealth.edu

Research Interest: My research is focused on understanding the role of mitochondrial dysfunction, mitophagy and endoplasmic reticulum (ER) stress in the development of lung diseases such as acute respiratory distress syndrome (ARDS) and bronchopulmonary dysplasia (BPD).

Obesity is a risk factor for developing ARDS. I am elucidating the mechanisms of how diet-induced obesity promotes endothelial dysfunction and primes endothelium to injury in response to various pulmonary insults such as bacteria/virus/sepsis/trauma/hyperoxia. Our main goal is to identify the metabolic targets that can be exploited for developing novel treatments to prevent this devastating complication seen after acute illness or injury.

My other focus is to understand the crosstalk between hyperoxia-induced mitochondrial dysfunction and cell stress in the development of BPD. We utilize genetic, molecular, and cellular experimental approaches that include fetal lung epithelial/endothelial cell lines, mice and human lung tissues to answer these questions.

Publications

Shah D, Torres Claudio and Bhandari V. Adiponectin deficiency induces mitochondrial dysfunction and promotes endothelial activation and pulmonary vascular injury. FASEB J. 2019 Dec;33(12):13617-13631

Shah D, Das P, Mohammad AA, Mahajan N, Romero F, Shahid M, Singh H, and Bhandari V. MicroRNA-34a promotes endothelial dysfunction and mitochondrial-mediated apoptosis in murine models of acute lung injury. Am J Respir Cell Mol Biol. 2019 Apr;60(4):465-477

Shah D, Syed MA, Das P, Andersson S, Pryhuber G, Bhandari V. TREM-1 Attenuates RIPK3 Mediated Necroptosis in Hyperoxia Induced Lung Injury in Neonatal Mice. Am J Respir Cell Mol Biol. 2019 Mar;60(3):308-322